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. 2018 Mar 19;19(3):902. doi: 10.3390/ijms19030902

Figure 1.

Figure 1

Loss of agnoprotein increases BK gene expression. (A) Schematic illustration of the BK Dunlop genome including the agnoprotein sequence mutated to generate the ΔAgno virus. Agnoprotein start codon in bold and base changes underlined in red; (B) Lysates from RPTE cells transfected with BK WT and ΔAgno genomes were probed with antibodies against early (LT) and late (VP1-3 and agnoprotein) proteins. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was included as a protein loading control. Loss of agnoprotein correlated with increased expression of other virus protein products; (C) Levels of early (LT) and late (VP1) mRNA transcripts were measured from RPTE cells containing BK WT or ΔAgno genomes. Levels of virus transcript were increased in the absence of agnoprotein; (D) Virus genome replication was measured by qPCR in RPTE cells containing BK WT and ΔAgno virus. Genome replication was increased in the absence of agnoprotein. All experiments are representative of at least three independent experimental repeats. Significance of changes were analyzed by Student’s t-test and indicated by * p <0.05, ** p <0.01.