Figure 4.

The possible pathway for the propagation of disturbance by residue mutation at Q74 in IL-2. (a) The dynamic network in IL-2 deduced by cross-correlation analysis. The dominant correlated pattern of the residues in IL-2 is shown relative to residues Q74. The residues color coded red, orange and yellow are correlated, but these residues are anticorrelated with those color-coded blue; (b) The dynamic network in IL-2 deduced by COREX/BEST algorithm. The energetic coupled regions in the network for Q74A are colored in red. For clarity the coupling regions only consist of the residues with the free energy change greater than 1.0 kcal/mol; (c) The possible allosteric pathway between the mutation site of Q74 and the coupling regions. Network representation of IL-2. The red bold edges represent allosteric pathways connecting mutation sites (magenta nodes) and coupling residues (blue nodes). The other components are represented as gray nodes.