Table 2.
Reagents used in immunofluorescence method.
| Primary Antibody | ||||
| Antisera | Code | Host Species | Dilution | Supplier |
| PGP9.5 | 7863-2004 | Mouse | 1:1000 | Bio-Rad, Hercules, CA, USA |
| CART | H-003-61 | Rabbit | 1:16,000 | Phoenix Pharmaceuticals, Inc., Burlingame, CA, USA |
| GAL | AB2233 | Guinea Pig | 1:2000 | EMD Millipore, Burlington, MA, USA |
| SP | 8450-0505 | Rat | 1:1000 | Bio-Rad |
| VAChT | H-V007 | Rabbit | 1:2000 | Phoenix Pharmaceuticals |
| VIP | 9535-0204 | Mouse | 1:2000 | Bio-Rad |
| Secondary antibodies | ||||
| Reagent | Dilution | Supplier | ||
| AF 488 donkey anti-mouse IgG (H + L) | 1:1000 | Thermo Fisher Scientific, Waltham, MA, USA | ||
| AF 546 goat anti-guinea pig IgG (H + L) | 1:1000 | Thermo Fisher Scientific | ||
| AF 546 goat anti-rabbit IgG (H + L) | 1:1000 | Thermo Fisher Scientific | ||
| AF 546 goat anti-rat IgG (H + L) | 1:1000 | Thermo Fisher Scientific | ||
PGP9.5 (pan-neuronal marker); CART, cocaine- and amphetamine-regulated transcript; GAL, galanin; SP, substance P; VAChT, vesicular acetylcholine transporter; VIP, vasoactive intestinal peptide; AF, Alexa Fluor.