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. Author manuscript; available in PMC: 2018 Mar 30.
Published in final edited form as: FEBS Lett. 2015 Jul 18;589(18):2297–2303. doi: 10.1016/j.febslet.2015.07.009

Fig. 4.

Fig. 4

Representative isothermal titration calorimetry (ITC) data of CtCBM3acipA-M5 titrated with carbohydrates. The soluble ligand (10 mg/mL in the syringe) was titrated into 54 μM CtCBM3acipA-M5 in the cell. In the case of cellulose the CBM was titrated into cellulose in the cell. The top half of each titration shows the raw injection heats; the bottom half, the integrated peak areas fitted using a single-site model (MicroCal Origin v7.0). ITC was carried out in 50 mM Na-HEPES pH 7.5 at 25°C.