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. Author manuscript; available in PMC: 2018 Jul 12.
Published in final edited form as: J Am Chem Soc. 2017 Jun 29;139(27):9128–9131. doi: 10.1021/jacs.7b04547

Figure 2.

Figure 2

Stability analysis of cb-Sgc8 and Sgc8 after treatment with Exo I. (a) Stability analysis of Sgc8 (upper) and cb-Sgc8 (lower) after incubation in 0.25 U/μL Exo I for different times, as determined by agarose electrophoresis. (b) Binding ability analysis of Sgc8 and cb-Sgc8 after treatment with Exo I, as determined by flow cytometry. The peak represents the fluorescence intensity of CCRF-CEM cells treated with library, Sgc8 (1), Sgc8 incubated in 0.25 U/μL Exo I for 1 h (2), Sgc8 incubated in 0.5 U/μL Exo I for 1 h (3), cb-Sgc8 (4), cb-Sgc8 incubated in 0.25 U/μL Exo I for 1 h (5), and cb-Sgc8 incubated in 0.5 U/μL Exo I for 1 h (6). The concentrations of Lib, Sgc8 and cb-Sgc8 in the flow cytometric assay were 10, 10 and 5 nM, respectively.