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. Author manuscript; available in PMC: 2018 Mar 30.
Published in final edited form as: Nat Protoc. 2017 May 18;12(6):1229–1244. doi: 10.1038/nprot.2017.034

Figure 3.

Figure 3

Chemoenzymatic sequential release of N-glycans and O-glycans from bovine-serum-derived fetuin using GIG. (i) N-glycans are released from the solid phase by PNGase F. (ii) O-glycans are cleaved using ammonium hydroxide (mild β-elimination) in the presence of 0.5 M PMP (1-phenyl-3-methyl-5-pyrazolone). (iii) Any remaining N-glycans are released by further PNGase F digestion60. Image adapted with permission from ref. 60, BioMed Central (open-access license: https://creativecommons.org/licenses/by/4.0/).