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. Author manuscript; available in PMC: 2018 Mar 30.
Published in final edited form as: Nat Protoc. 2017 May 18;12(6):1229–1244. doi: 10.1038/nprot.2017.034

Figure 4.

Figure 4

Sialylated O-glycans of mucin from bovine submaxillary glands by MALDI-MS. (a) The sialic acids that were stabilized by carbodiimide coupling have a substantially increased MS signal. (b) The sialic acids without modification have low intensity in MALDI-MS. An internal standard (neurotensin (20 μM/1 μl) was spiked into the sample. The sialic-acid-modified glycans have one sodium adduct [Na]+, whereas native glycans have an extra sodium adduct per sialic acid60. Image adapted with permission from ref. 60, BioMed Central (open-access license: https://creativecommons.org/licenses/by/4.0/).