|
| ||
| Item | Parameter | Value |
|
| ||
| LC pump | Loading pump (μl/min) | 5 |
| Separation (Nanoflow pump) (μl/min) | 0.25 | |
| LC gradient | Linear gradient of 4-50% ACN (0.1% TFA) (min) | 70 |
| MS1 | MS1 mass range (Da) | 400-1,800 |
| MS2 | Collision energy (%) | 29 |
| Isolation width (m/z) | 2 | |
| Activation time (ms) | 0.2 | |
| High-energy collision dissociation (HCD) | On | |
| Repeat count (dynamic exclusion) | 2 | |
| Repeat duration (dynamic exclusion) (s) | 25 | |
| Exclusion list size (dynamic exclusion) | 500 | |
| Exclusion duration (dynamic exclusion) (s) | 5 | |
|
| ||
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