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. 2001 Sep 18;98(20):11615–11620. doi: 10.1073/pnas.211330598

Figure 6.

Figure 6

Analysis of the interactions between TorA and TorC or apoTorC proteins. (A) the sensorgrams of interactions between immobilized TorA and TorC (solid line) or apoTorC (dotted line) are expressed in resonance units (RU). Proteins (50 μl at 1 μM) were injected into a sensor chip with dextran matrix coupled either to TorA or to no protein as a control. The control flow cell (no protein immobilized) was subtracted from the experimental flow cells. The arrow indicates the end of the injection. (B) Cross-link of TorA and TorC or apoTorC. TorA (5 μM) was incubated with EDC (1 mM) in the presence of either TorC (5 μM) or apoTorC (5 μM) for 30 min at room temperature. Samples were loaded on an SDS/12.5% polyacrylamide gel. After electrophoresis, proteins were visualized by Western blotting with anti-TorA antibodies. The arrow indicates a complex between TorC and TorA.