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. Author manuscript; available in PMC: 2018 Mar 30.
Published in final edited form as: Toxicology. 2015 Jan 9;329:32–39. doi: 10.1016/j.tox.2015.01.007

Fig. 3.

Fig. 3

Isoflavone-induced enhancement of RORα- and RORγ-mediated Il17a promoter activation in Jurkat cells. Cells were co-transfected with the pCMV-β-Gal and pCMV10-3xFlag-RORα (A) or pCMV10-3xFlag-RORγ (B) and pGL4.14 reporter plasmid under the control of the Il17a promoter and treated with increasing concentrations of the isoflavones at 0.1, 1, and 10 μM. After 24 h, relative LUC activity was determined as described in Materials and Methods. The firefly luciferase activity was normalized against β-galactosidase activity. BA, GE, FN, and DZ represent each isoflavone, biochanin A, genistein, formononetin, and daidzein, respectively. Values represent the means ± SEM (n = 3). Significant differences from the vehicle control (DMSO) plus RORα or RORγ are indicated by asterisks (*P < 0.05; 1-way ANOVA).