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. Author manuscript; available in PMC: 2018 Mar 30.
Published in final edited form as: Cell Rep. 2018 Mar 6;22(10):2756–2766. doi: 10.1016/j.celrep.2018.02.039

Figure 4. CRF Interacts with Phenylephrine to Drive LTP without IP3-Induced Ca2+ Signal Facilitation.

Figure 4

(A) Summary time graph (left) and example traces (right) showing that CRF augments facilitation of AP-evoked IK(Ca) produced by a low concentration (0.5 μM) of phenylephrine (7 cells from 5 rats).

(B) Graph plotting the magnitude of IK(Ca) facilitation caused by phenylephrine (0.5 μM) alone and by phenylephrine and CRF in individual cells; t(6) = 2.22, *p < 0.05, two-tailed paired t test.

(C) Representative experiment to induce LTP in the presence of both CRF and phenylephrine (0.5 μM) using an induction protocol consisting of synaptic stimulation-burst pairing with no preceding IP3 application. Example EPSC traces at the times indicated are shown in inset (scale bars: 50 ms/50 pA).

(D) Summary time graph of LTP experiments in which LTP was induced using a synaptic stimulation-burst pairing protocol in the presence of both CRF and phenylephrine (0.5 μM) (7 cells from 4 rats).

Data are presented as mean ± SEM.