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. Author manuscript; available in PMC: 2018 May 6.
Published in final edited form as: Nat Struct Mol Biol. 2017 Nov 6;24(12):1057–1063. doi: 10.1038/nsmb.3499

Figure 1.

Figure 1

The C. elegans poly(A) profile. (a) Outline of the adapted mTAIL-seq procedure. A splint oligo is used to select for polyadenylated RNAs and exclude other RNA contaminants. (b and c) Global size distribution of C. elegans poly(A) tails measured by mTAIL-seq (b) and bulk poly(A) labeling (c). (d) Distribution of median poly(A) tail-length per gene (n = 13,601 protein coding genes). Genes with a median tail ≤ 70 nt were categorized as short-tailed (n = 3,570), genes with a median tail >70 and ≤ 94 nt were categorized as medium-tailed (n = 6,648) and genes with a median tail > 94 nt were categorized as long-tailed (n = 3,383). (e) Functional annotations (Gene Ontology terms) significantly enriched for genes with short or long tails. The colored bars represent the percent of members in each tail-length category. (f) Tissue enrichment profiles for genes with short, medium or long tails. ▲ significant enrichment; ▼ significant depletion for a tissue category (p<0.01, Fisher test). Poly(A) tail measurements, DAVID Gene Ontology Analysis, and tissue enrichment analysis for C. elegans transcripts are available in Supplementary Data Set 1.