Table 1.
Sr. no. | Size of meristem (mm) | Degree of phenolic exudation on medium | Meristems died due to desiccation (%) | Meristems died due to contamination (%) | Percent establishment | No. of explants turned to callus | No. of explants proliferated | No of shoots developed per explant |
---|---|---|---|---|---|---|---|---|
1. | 0.2–0.3 | − | 48.91 (44.38) |
31.52 (34.14) |
20.65 (27.00) |
14 | – | – |
2. | 0.3–0.4 | − | 37.14 (37.55) |
37.14 (39.49) |
25.71 (30.44) |
11 | 3 | 1–2 |
3. | 0.4–0.5 | − | 25.64 (30.41) |
42.30 (40.57) |
32.05 (34.48) |
14 | 6 | 1 |
4. | 0.5–0.6 | + | 17.07 (24.38) |
47.56 (43.60) |
32.92 (35.01) |
16 | 6 | 1 |
5. | 0.6–0.7 | ++ | 12.50 (20.70) |
48.86 (44.35) |
36.36 (37.08) |
17 | 9 | 1–2 |
S.E. | 1.48 | 2.76 | 1.61 | |||||
C.D.(0.05) | 3.31 | 6.15 | 3.60 |
Values in parenthesis are arc sine transformed values
S.E. standard error, C.D. critical difference, very less phenols; + less phenols; ++ more phenols