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. 2018 Mar 30;14(3):e1006945. doi: 10.1371/journal.ppat.1006945

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© 2018 Deo et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — (A, B and C) Bone marrow-derived macrophages (BMDMs) were treated with purified N. gonorrhoeae OMVs for 24 hours and imaged by transmission electron microscopy by negative staining and (C) 10 nm-gold conjugated antibodies against PorB. (D) Crude mitochondria (P) and cytoplasmic fractions (S) were obtained from OMV-treated BMDMs and analyzed for PorB and BamA and mitochondria (Tim23, Tom40 and F₁β) and cytosolic (GAPDH) markers by immunoblot analysis after semi-native gel electrophoresis. (E) Mitochondria-enriched fractions were incubated with purified OMVs or PBS and subsequent supernatant (S) and pellet (P) fractions were analyzed by immunblotting with anti-PorB, BamA and Tim23 antibodies after SDS gel electrophoreses.