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. 2018 Mar 30;14(3):e1006945. doi: 10.1371/journal.ppat.1006945

Fig 7. N. gonorrhoeae depends on OMVs for PorB delivery to macrophages.

Fig 7

(A) Wild type (WT) and ΔNGFG_01788 deletion mutant were cultured in GC media and growth was monitored by optical density at 600 nm. Mean and standard deviation from triplicate experiments. (B) Transmission electron microscopy of ΔNGFG_01788 mutant showing diplococci and OMV secretion. (C) Purified OMVs and whole bacteria lysates were analyzed by coomassie staining after SDS gel electrophorese and the amount of PorB within OMVs relative to whole bacterial cells determined by densitometry. Relative OMV-mediated secretion of PorB in the mutant is based on wild type levels (100%). Mean and standard deviation from three independent experiments are shown. (D) BMDMs were incubated with wild type N. gonorrhoeae or (E) ΔNGFG_01788 mutant in transwells and analyzed for PorB (red) and Tom20 (green) localization by immunofluorescence analysis after 24 hours. DAPI (blue) indicates cell nucleus. (F) The number of PorB signals (puncta) was quantified from >100 cells and two independent experiments.