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. 2018 Feb 20;115(10):E2183–E2192. doi: 10.1073/pnas.1714530115

Fig. 4.

Fig. 4.

Single-molecule superresolution imaging of OligoMiner oligos. (A and B) Diffraction-limited (A) and superresolved STORM (B) images of a probe set consisting of 3,678 LDM oligos targeting 1,035 kb at 19p13.2 in human XY 2N PGP-1 fibroblasts. (C and D) Diffraction-limited (C) and superresolved STORM (D) images of a probe set consisting of 104 LDM oligos targeting 20 kb at 19p13.2 in PGP-1 fibroblasts. (E and F) Diffraction-limited (E) and superresolved DNA-PAINT (F) images of a probe set consisting of 4,776 UM oligos targeting 817 kb at Xq28 in human XY 2N MRC-5 fibroblasts. (G and H) Diffraction-limited (G) and superresolved DNA-PAINT (H) images of a probe set consisting of 176 LDM oligos targeting 11 kb of the Xist RNA in human XX 2N WI-38 fibroblasts. (i–viii) Normalized single-molecule counts along the indicated 1D line traces (blue bars) and one- or two-component Gaussian fits to the underlying data (black lines). Superresolution data are presented using a “hot” color map in which single-molecule localization density scales from black (lowest) to red to yellow to white (highest). (Scale bars: 500 nm.) The minimum and maximum values of detected photons per square nanometer used to set the display scale is shown to right of each superresolution image, and the SD of the Gaussian blur used in the construction of each superresolution image is denoted in the top right corner.