Fig. 1.

Analyze the development of postnatal AT1 cells by single-cell RNA-seq (scRNA-seq). (A) Schematic illustration of the strategy of lung dissociation, AT1 cell sorting, and single-cell RNA sequencing analysis. (B–D) RFP⁺Pdpn⁺GFP⁻CD45⁻ cells were isolated from TAM-treated Sftpc-CreER; Rosa26-Zsgreen; Hopx-tdTomato mice at P3 (B), P15 (C), and P60 (D). The t-distributed stochastic neighbor embedding plots show cells isolated from P3 (B), P15 (C), and P60 (D) lungs can be clustered into four, four, and two main distinct populations, respectively. AT1 cell population is characterized by expressing Hopx and Pdpn (AT1 markers), but not Sftpc (AT2 marker), Pecam1 (endothelial cell marker), and Foxj1 (ciliated cell marker).