Fig. 3.

Tim-3 is not required for the development of functional T cell exhaustion. (A) Mice were infected with 2 × 10⁵ PFU LCMV-Cl13 and weighed throughout the course of infection. Uninfected, n = 2; WT, n = 5; Tim-3 KO, n = 6. *P ≤ 0.01, two-tailed unpaired Student’s t test between WT and KO at that time point (mean ± SD). (B) At day ≥30, mice from A were killed, and virus in the blood was measured by qPCR using a plasmid standard curve. Data are presented as mean ± SEM. (C) Tetramer⁺ cells in the spleen were quantified using pooled LCMV tetramers (GP33, GP276, and NP396); each point represents an individual mouse (mean ± SD). Data are representative of three independent experiments. (D) Splenocytes were stimulated with pooled LCMV peptides. Each point represents an individual mouse (mean ± SD) pooled from three independent experiments. (E) Splenocytes were fixed and permeabilized for staining with Abs to Tbet and Eomes. Each point represents an individual mouse. Data (mean ± SD) are representative of three independent experiments. In C and D, *P < 0.05, **P < 0.01, two-tailed unpaired Student’s t test.