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. 2018 Feb 21;115(10):E2348–E2357. doi: 10.1073/pnas.1707337115

Fig. 2.

Fig. 2.

Exacerbation of aging traits in OFO. (A) Experimental design. (B) Nitrotyrosine abundance, as determined by Western blot, in brain homogenates of aged YFO and OFO animals (old YFO, n = 6 mice from six litters; old OFO, n = 6 mice from six litters) and young adult reference groups (young YFO, n = 6 mice from six litters; young OFO, n = 6 mice from six litters) (age: P < 0.0001; paternal age: P = 0.006; interaction: P = 0.30). (C–N) Histopathological aging traits examined in aged YFO and OFO animals (old YFO, n = 37 mice from 12 litters; old OFO, n = 45 mice from 17 litters) as well as young adult reference groups (young YFO, n = 16 mice from six litters; young OFO, n = 14 mice from seven litters). Shown are examples of histological images, probability maps (generated via automated segmentation of histological images), and if applicable, primary object identification maps, along with the quantification of the respective image features. (C) Bronchus-associated lymphoid tissue (age: P < 0.0001; paternal age: P = 0.02; interaction: P = 0.10); (D) thyroid follicle morphology (form factor; a value of 1 reflecting a perfectly circular structure; values between 1 and 0 report progressively more irregularly shaped structures) (age: P < 0.0001; paternal age: P < 0.0001; interaction: P = 0.18); (E) age-related gland-like structures in the tracheal submucosa (age: P = 0.0008; paternal age: P = 0.02; interaction: P = 0.10); (F) skeletal muscle fiber atrophy (age: P < 0.0001; paternal age: P = 0.007; interaction: P = 0.82); (G) macrovesicular lipidosis affecting the liver (age: P < 0.0001; paternal age: P = 0.14; interaction: P = 0.02); (H) liver microgranulomas (age: P = 0.18; paternal age: P = 0.18; interaction: P = 0.35); (I) myocardial fibrosis (age: P = 0.02; paternal age: P = 0.08; interaction: P = 0.13); (J) elastic fiber content of arterial walls (age: P < 0.0001; paternal age: P = 0.02; interaction: P < 0.0001); (K) testis atrophy (age: P = 0.004; paternal age: P = 0.03; interaction: P = 0.55); (L) renal tubular atrophy (age: P < 0.0001; paternal age: P = 0.046; interaction: P = 0.001); (M) renal vascular hyalinosis (age: P = 0.0003; paternal age: P = 0.27; interaction: P = 0.27); (N) glomerulosclerosis (age: P = 0.0002; paternal age: P = 0.01; interaction: P = 0.02). [Scale bars: 1 mm (C and I); 300 µm (D and G); 100 µm (E, F, and H–N).] *P < 0.05; **P < 0.01; ***P < 0.001. Graphs show mean ± SEM.