Fig. 2.

CT slices and histogram distributions of the same whole mouse kidney before and after staining highlighting the contrast enhancement obtained after application of the eosin-based staining protocol. Both data sets were acquired with the Xradia Versa 500 microCT using identical acquisition parameters. The voxel size in both data sets is 12 µm. (A) Overview image of the unstained mouse kidney. (B) Overview image of the same mouse kidney sample shown in A after staining. The following anatomic structural regions could be identified and were labeled: cortex (B, I), outer medulla (B, II) with further distinction in outer stripes of outer medulla (B, IIa) and inner stripes of outer medulla (B, IIb), inner medulla (B, III), papilla (B, IV), and renal pelvis (B, V). (C) Histogram of CT slice shown in A, and (D) Histogram of CT slice shown in B.