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. Author manuscript; available in PMC: 2018 Mar 30.
Published in final edited form as: Cell Biol (Henderson, NV). 2017 Jun 19;6(2):133. doi: 10.4172/2324-9293.1000133

Figure 4.

Figure 4

Effects of S/T→A mutations on ASPH protein expression and subcellular localization: PNET2 cells were transiently transfected with wildtype (WT) or a pointmutated (M#:S/T→A) N-Myc-ASPH cDNA. Myc-empty vector (EV) served as a negative control. The M19-H675Q mutant, disrupting ASPH’s catalytic activity, served as a positive control. Representative results obtained by immunofluorescence staining and confocal imaging of cells transfected with (A) WT, (B) M7-S24A, (C) M18-T748A, (D) M19-H675Q, or (E) EV and stained by immunofluorescence with anti-Myc. Immunoreactivity was detected with biotinylated secondary antibody and Streptavidin-conjugated Dylight 547 (red). Cells were counterstained with DAPI (blue). (Merged images: 600× magnification, 2× digital zoom).