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. Author manuscript; available in PMC: 2018 Mar 30.
Published in final edited form as: Cell Biol (Henderson, NV). 2017 Jun 19;6(2):133. doi: 10.4172/2324-9293.1000133

Figure 6.

Figure 6

Effects of WT and point mutated ASPH on Notch signaling genes: PNET2 cells transfected with WT or point-mutated N-Myc-ASPH cDNAs were used in duplex qRT-PCR assays to measure (A) Notch-1, (B) Jagged-1, (C) HES-1, and (D) HEY-1 expression. Data were normalized to HPRT measured simultaneously in the same reactions. EV-transfected cells served as negative controls, whereas M19-H◊Q transfected cells served as positive controls for inhibiting Notch signaling due to loss of catalytic activity. Graph depict relative mean (± S.D.) levels of target gene (mRNA) expression. Inter-group comparisons were made by one-way ANOVA with the post-hoc Fisher LSD test. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001 for comparisons with EV (S-Table 3).