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. Author manuscript; available in PMC: 2019 Jul 1.

Published in final edited form as: J Biomed Mater Res B Appl Biomater. 2017 Sep 30;106(5):1964–1975. doi: 10.1002/jbm.b.34010

(a) Representative fluorescence microscopy images of adhered platelets and leukocytes stained with Calcein-AM on PS, LLDPE, and HA-LLDPE surfaces.

(b) Percent coverage analysis of adhered platelets and leukocytes after staining with Calcein-AM on PS, LLDPE, and HA-LLDPE surfaces. Percent coverage of platelets were determined by the evaluation of 15 representative images taken per group over 3 studies (n = 45) using ImageJ. Each image covered an area of 25 µm². Results indicated significantly less cellular adhesion on HA-LLDPE as compared to other surfaces (p ≤ 0.05).

(a) Representative fluorescence microscopy images of adhered platelets and leukocytes stained with Calcein-AM on PS, LLDPE, and HA-LLDPE surfaces.

(b) Percent coverage analysis of adhered platelets and leukocytes after staining with Calcein-AM on PS, LLDPE, and HA-LLDPE surfaces. Percent coverage of platelets were determined by the evaluation of 15 representative images taken per group over 3 studies (n = 45) using ImageJ. Each image covered an area of 25 µm². Results indicated significantly less cellular adhesion on HA-LLDPE as compared to other surfaces (p ≤ 0.05).