Fig. 2.

OxPAPC induces expression of cell-associated and secreted SCF protein and stimulates c-Kit phosphorylation. a Two forms of SCF mRNA were quantified in OxPAPC-stimulated HUVECs (100 µg/ml, 6 h) by qRT-PCR using primers selective for individual splice variants. b SCF protein was analyzed by ELISA in extracts of HUVECs or in conditioned media after 12 h of stimulation with 100 µg/ml OxPAPC. c Conditioned media produced by cells stimulated with indicated concentrations of OxPAPC stimulate phosphorylation of c-Kit receptor in HUVECs. d Panel presents ELISA data on the levels of SCF protein in conditioned media. e Pretreatment of cells with c-Kit tyrosine kinase inhibitor imatinib (30 min, 20 µM) attenuated c-Kit phosphorylation in HUVECs incubated with conditioned media from OxPAPC-stimulated cells