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. 2018 Jan 13;21(2):267–285. doi: 10.1007/s10456-018-9594-9

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Effects of IKK2 inhibition on CCL2 and CXCL5 expression in HUVEC under TNFα stimulation. a Immunofluorescence images of CCL2 (green) and CXCL5 (green) treated with IMD0354 and stimulated by recombinant human TNF-α (rhTNFα). DMSO and rhTNF-α treatment were used as a negative and positive control, respectively. Cell nuclei visualized with DAPI staining (blue). Quantitative qRT-PCR analysis of b CCL2 and c CXCL5 expression in IMD0354 treated and rhTNF-α stimulated HUVEC (n = 4). One-way ANOVA test with Tukey multiple comparisons was used to determine statistical significance. *p < 0.05; ***p < 0.001