Fig. 1.

Docetaxel induces cell apoptosis and autophagy in PC3 and DU145 cells. a PC3 and DU145 cells were treated with various concentrations of Doc for 12 h, and cell extracts were analyzed to determine changes in protein expression by western blot analysis. b PC3 and DU145 cells were treated with Doc (100 ng/ml) for the indicated time. Western blot was used to detect protein expression. c PC3 cells were treated with Doc (100 ng/ml) for 12 h, and LC3 punctate formation was assayed by confocal microscopic analysis. Images are representative of 10 random fields. d PC3 and DU145 cells were cultured in Doc (100 ng/ml) for 12 h with or without CQ pretreatment (10 μM, 30 min). Cell extracts were analyzed for protein expression using western blot analysis. *P < 0.05, **P < 0.01