Fig. 2.

Docetaxel induces autophagy by JNK signaling pathway activation in PC3 and DU145 cells. a PC3 cells were cultured in Doc (100 ng/ml) for 12 h with different inhibitor (10 μM, 30 min). Cell extracts were analyzed for protein expression using western blot analysis. b PC3 and DU145 cells were treated with Doc (100 ng/ml) for the indicated time, western blot was used to detect protein expression. c PC3 cells were cultured in Doc (100 ng/ml) for 4 h with or without SP600125 pretreatment (10 μM, 30 min), LC3 punctate formation was assayed by confocal microscopic analysis. Images are representative of 10 random fields. d PC3 and DU145 cells were cultured in Doc (100 ng/ml) for 4 h with or without SP600125 pretreatment (10 μM, 30 min), western blot was used to detect protein expression. *P < 0.05, **P < 0.01