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. 2018 Apr 1;15(2):188–201. doi: 10.1089/zeb.2017.1528

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Copyright 2018, Mary Ann Liebert, Inc.

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FIG. 4. — P. neurophilia 2014 cryopreservation results. The ZIRC sperm cryopreservation method used at that time was used in this assay. Each bar is the average spore survival based on SYTOX (white bars), Fungi-Fluor (gray bars), or presence of spore vacuoles (striped bars). Black dots represent each of the replicate samples. All of the other samples contained only spores with distilled water. The 4°C+ZIRC-S samples contained spores, zebrafish sperm, and the freezing medium (Ginsburg Fish Ringers), but they were kept at 4°C. ZIRC, without sperm; ZIRC-S, cryopreservation with sperm.