FIG. 5.

P. neurophilia 2017 cryopreservation results. The current ZIRC sperm cryopreservation method (2017) was used in this assay. Each bar is the average spore survival based on SYTOX (white bars), Fungi-Fluor (gray bars), or presence of spore vacuoles (striped bars). Each black dot equals one of three replicate samples. There was a significant decrease in the percentage of spores scored alive by the presence of vacuoles or by Fungi-Fluor in the ZIRC samples compared with the 20°C samples. (Two-sample t-test, p = 0.005.)