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. Author manuscript; available in PMC: 2018 Mar 31.
Published in final edited form as: Methods Mol Biol. 2016 Apr 10:10.1007/7651_2016_352. doi: 10.1007/7651_2016_352

Table 1.

Fluorescence readings from the initial photos

Untreated control cells ATO 5 µM, 72 h

ex. 810 nm ex. 488 nm ex. 810 nm ex. 488 nm
Area Mean IntDen Area Mean IntDen Area Mean IntDen Area Mean IntDen
Whole 21,024 10.99 2,31,092 21,315 2.38 50,696 21,608 11.86 2,56,181 21,316 1.74 37,067
Blank 1 341 5.71 1946 366 1.09 398 354 7.54 2670 75 1.44 108
Blank 2 214 5.65 1210 432 0.99 429 238 6.52 1552 147 1.01 149
Blank 3 412 5.54 2284 320 1.00 320 100 6.26 626 140 1.09 152
Cell 1 642 31.02 19,913 435 5.52 2402 620 36.96 22,918 216 11.6 2506
Cell 2 506 26.31 13,313 638 15.22 9708 326 44.87 14,626 311 7.26 2259
Cell 3 452 24.04 10,867 690 6.94 4792 253 43.82 11,086 586 4.87 2856
Cell 4 376 25.15 9457 561 5.69 3191 359 40.03 14,369 535 3.26 1743
Cell 5 402 16.89 6788 550 4.17 2296 364 28.25 10,283 177 8.02 1420