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. Author manuscript; available in PMC: 2018 Mar 31.
Published in final edited form as: Methods Mol Biol. 2016 Apr 10:10.1007/7651_2016_352. doi: 10.1007/7651_2016_352

Table 2.

Details on calculation of fluorescence ratio

Control ATO
810 nm 488 nm 810 nm 488 nm
Mean-whole image 10.99 2.38 11.86 1.74
Actual mean-whole image 5.36 1.35 5.09 0.56
Area-whole 21,024 21,315 21,608 21,316
Total fluorescence-whole 1,12,618.56 28,846.30 1,09,912.69 11,936.96
Mean-blank 5.63 1.03 6.77 1.18
Mean-5 cells 24.682 7.51 38.79 7.00
Mean-5 cells actual 19.05 6.48 32.01 5.82
Total area-5 cells 2378 2874 1922 1825
Total fluorescence-5 cells 45,297.73 18,627.35 61,528.35 10,625.20
Fluorescence per cell 9059.55 3725.47 12,305.67 2125.03
810/488-whole 3.90 9.21
810/488 per cell 2.43 5.79
Fold of increase-whole 2.36 ATO/Ctrl
Fold of increase-per cell 2.38 ATO/Ctrl