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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Trends Genet. 2018 Jan 21;34(4):291–300. doi: 10.1016/j.tig.2017.12.017

Figure 2. PRDM9 binding, nucleosome modification, repositioning, and DSB initiation.

Figure 2

PRDM9 binding sites can be determined in vitro (Affinity-seq, red [45]) and in vivo (PRDM9 ChIP-seq, blue [47]). Its subsequent histone trimethylation at nearby nucleosomes (H3K4me3, blue; H3K36me3, orange [35]) limits the space in which double strand break initiation will occur (DMC1, purple [49]). The nucleosome repositioning after PRDM9 trimethylation of H3K4 and H3K36 is presented above the main panel.