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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Angiogenesis. 2018 Jan 11;21(2):215–228. doi: 10.1007/s10456-017-9589-y

Fig. 4. Ligation of IL-11 to IL-11Rα induces RA ST fibroblasts migration.

Fig. 4

A. Effect of IL-11 (1–200ng/ml) on RA ST fibroblast migration was examined using an in vitro scratch assay for 24h. B. Representative images of RA ST fibroblast scratch assays performed on untreated cells (PBS) or cells treated with IL-11 (100ng/ml) with or without IL-11Rα-Fc chimera (10μg/ml, IL-11 antagonist). Additionally, supernatants obtained from RA ST fibroblasts that were untreated (PBS sup) or stimulated for 48h with IL-11 (100ng/ml) were tested in presence or absence of IL-11Rα-Fc chimera (10μg/ml). C. Number of fibroblasts counted in the scratch area shown in B. D. Representative images of the RA fibroblast scratch assay performed for 24h using the supernatants from HUVECs that were untreated (PBS sup) or treated with IL-11 (100ng/ml) in the absence or presence of IL-11Rα-Fc chimera (added 1h prior to use). E. Number of fibroblasts counted in the scratch area shown in D. F. MTT assay was performed in RA ST fibroblasts that were untreated (PBS) or treated with 100ng/ml of IL-11 or bFGF for 24–96h. OD was measured at 570 nm and the data in each time point is shown as fold increase above control. In all the experiments, bFGF (100ng/ml) was used as a positive control. All treatments were done in triplicate and each experiment was performed 3 independent times. Values are the mean ± SD. * represents p <0.05.