(A) Schematic diagram of the construct used for generation of
cGsαR201H transgenic mice. The Gsa-R201H cDNA, as well as
the lacZ gene, is expressed upon Cre recombinase activity. (B)
Detection of β-galactosidase activity in BMSCs upon adenovirus
transduction. Four to six-week-old F1 or F2 generation mice from each of the
five founder lines were used for validation. Results are shown for lines
#6, #9, and #18 only. Ad-LacZ was used
as positive control for the staining. NT, non-transduced. Ad-YFP was used as
negative control. Note that lines #6 and #18, but not line
#9, were able to express the transgene upon Cre recombinase
activity.