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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Bone. 2018 Feb 20;110:230–237. doi: 10.1016/j.bone.2018.02.016

Figure 1. Generation and validation of transgenic mice conditionally expressing a constitutively active Gsα mutant.

Figure 1

(A) Schematic diagram of the construct used for generation of cGsαR201H transgenic mice. The Gsa-R201H cDNA, as well as the lacZ gene, is expressed upon Cre recombinase activity. (B) Detection of β-galactosidase activity in BMSCs upon adenovirus transduction. Four to six-week-old F1 or F2 generation mice from each of the five founder lines were used for validation. Results are shown for lines #6, #9, and #18 only. Ad-LacZ was used as positive control for the staining. NT, non-transduced. Ad-YFP was used as negative control. Note that lines #6 and #18, but not line #9, were able to express the transgene upon Cre recombinase activity.