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. 2001 Sep 18;98(20):11674–11679. doi: 10.1073/pnas.201398898

Figure 1.

Figure 1

Specificity of the DP antibody. (A) Western blot analysis with the DP antibody. The glutathione transferase-fused C-terminal fragment of DP expressed in E. coli (30 ng of protein, lane 1) and homogenate (200 μg of protein) of COS cells expressing receptor for DP (lanes 2, 5, and 6), EP2 (lane 3), or PGF (lane 4) were subjected to SDS/PAGE and transferred to a nitrocellulose membrane. The blot was immunostained with the DP antibody (lanes 1–4), normal guinea pig IgG (lane 5), or preadsorbed DP antibody (lane 6). (B) Immunofluorescence staining with the DP antibody. Photographs indicate the native CHO cells (b) and CHO cells stably expressing DP (a), EP2 receptor (c), or PGF receptor (d).