Table 2.
Longevity of unpollinated TFs silenced flowers on petunia
| Gene ID | Flower longevity (days ± SD) | Description | Increased/decreased days compared to WT | Increased/decreased rate of longevity compared to WT (%) | Reference |
|---|---|---|---|---|---|
| Experiment 1 | |||||
| Peaxi162Scf00081g00151 | 9.89 ± 1.43c | Zinc finger | 1.47* | 17.46 | |
| Peaxi162scf00013g00084 | 7.04 ± 1.08b | Zinc finger | −1.38 | −16.39 | |
| Peaxi162Scf00060g00211 | 7.93 ± 1.48b | Zinc finger | −0.49 | −5.82 | |
| Peaxi162Scf00105g01114 | 9.5 ± 1.57c | Zinc finger | 1.08* | 12.83 | |
| Peaxi162Scf00207g01365 | 9.17 ± 0.75c | Zinc finger | 0.75 | 8.91 | |
| Peaxi162Scf00003g00267 | 8.5 ± 1.06a | Zinc finger | 0.08 | 0.95 | |
| Peaxi162Scf00016g00288 | 9.95 ± 1.29c | Zinc finger | 1.53* | 18.17 | |
| Peaxi162Scf00013g00059 | 9.63 ± 0.69c | Zinc finger | 1.21* | 14.37 | |
| Peaxi162Scf00167g00083 | 10.7 ± 1.19d | ERF | 2.28** | 27.08 | |
| Peaxi162Scf00024g00271 | 9.64 ± 0.55b | ERF | 1.22* | 14.49 | |
| Peaxi162Scf00102g01713 | 8 ± 0.87a | ERF | −0.42 | −4.99 | |
| Peaxi162Scf00092g01520 | 9.67 ± 1.68c | Homeobox | 1.25* | 14.85 | |
| Peaxi162Scf00237g01510 | 10.15 ± 1.55d | Homeobox | 1.73* | 20.55 | [22] |
| Peaxi162Scf00285g00311 | 11.36 ± 1.23d | bHLH | 2.94** | 34.92 | [51] |
| Peaxi162Scf00712g00029 | 9.42 ± 1.46c | bHLH | 1* | 11.88 | |
| Peaxi162Scf00119g00942 | 8.41 ± 1.22a | bHLH | −0.01 | −0.12 | |
| Peaxi162Scf00428g01020 | 8.35 ± 0.77a | bHLH | −0.07 | −0.83 | |
| Peaxi162Scf00081g02226 | 8.79 ± 1.07a | LIM | 0.37 | 4.39 | |
| Peaxi162Scf01083g00019 | 10.25 ± 1.6d | MYB | 1.83* | 21.73 | |
| Peaxi162Scf00420g00728 | 8.4 ± 1.17a | NAM | −0.02 | −0.24 | |
| Peaxi162Scf00045g01824 | 8.73 ± 1.49a | CO-like | 0.31 | 3.68 | |
| Peaxi162Scf00314g00539 | 7.27 ± 0.88b | ARF2 | −1.15 | −13.66 | |
| Peaxi162Scf00341g00036 | 9.7 ± 1.46c | DNA binding | 1.28* | 15.20 | |
| TRV CHS white flowers | 8.68 ± 1.19a | Control 1 | |||
| WT purple flowers | 8.42 ± 1.14a | Control 2 | |||
| Experiment 2 | |||||
| Peaxi162Scf01105g00218 | 9.6 ± 0.77a | NAM | 0.1 | 1.05 | |
| Peaxi162Scf00051g01410 | 10 ± 1.15b | NAM | 0.5 | 5.26 | |
| TRV CHS white flowers | 9.35 ± 0.75a | Control 1 | |||
| WT purple flowers | 9.5 ± 0.79a | Control 2 | |||
| Experiment 3 | |||||
| Peaxi162Scf00998g00127 | 7.96 ± 0.96a | NAM | 0.75 | 10.40 | [3] |
| Peaxi162Scf00287g00193 | 8.5 ± 1.89a | ARF19 | 1.29* | 17.89 | |
| Peaxi162Scf00285g00011 | 5.6 ± 0.68b | bZIP | −1.61 | −22.33 | [54] |
| TRV CHS white flowers | 7.79 ± 0.92a | Control 1 | |||
| WT purple flowers | 7.21 ± 0.95a | Control 2 | |||
| Experiment 4 | |||||
| Peaxi162Scf00002g00037 | 9.8 ± 1.13a | MYB | 0.75 | 8.29 | |
| Peaxi162Scf00716g00024 | 9.07 ± 1.28a | bHLH | 0.02 | 0.22 | |
| Peaxi162Scf00128g01526 | 9.88 ± 1.38a | TCP | 0.83 | 9.17 | |
| Peaxi162Scf01084g00119 | 12.8 ± 2.9b | MADS | 3.75*** | 41.44 | |
| TRV CHS white flowers | 9.15 ± 1.55a | Control 1 | |||
| WT purple flowers | 9.05 ± 1.23a | Control 2 | |||
Petunia plants were infected with Agrobacterium transformed with pTRV2/CHS as an empty vector control (named TRV CHS white flowers) or pTRV2/CHS-TF vector (displayed as Gene ID). WT purple flowers as an uninfected control. For each line, 10 corollas from each of three individual plants were used for testing flower longevity. The recorded longevity represents the time from flower full open but before anthers dehiscing to complete wilting of the corolla. Flower longevity data (means ± SD, n = 10) followed by different letters are significantly different at P = 0.05 within a given experiment
Notes: *: extending 1 day more than WT; **: extending 2 days more than WT; ***: extending 3 days more than WT; −: days and % of WT decreased