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. Author manuscript; available in PMC: 2018 Dec 14.
Published in final edited form as: Cell. 2017 Nov 30;171(7):1611–1624.e24. doi: 10.1016/j.cell.2017.10.044

Figure 7. p-EMT predicts nodal metastasis and adverse pathologic features.

Figure 7

(A) PC1 and PC2 gene scores based on PCA of inferred malignant cell-specific profiles from all malignant-basal TCGA tumors (n=225). p-EMT genes (red) and epithelial differentiation genes (green) underlie variance among malignant-basal tumors.

(B) PC1 and PC2 gene scores based on PCA of inferred malignant cell-specific profiles from all classical and atypical TCGA tumors (n=156). p-EMT (red) and epithelial differentiation (green) genes are weakly associated with variance in these tumors.

(C) Plot depicts percentage of p-EMT high and p-EMT low malignant-basal tumors associated with each clinical feature. Higher p-EMT scores were associated with positive LNs, advanced nodal stage, high grade, extracapsular extension (ECE), and lymphovascular invasion (LVI) (hypergeometric test, p<0.05). Advanced local disease (T3/T4) as determined by T-stage did not correlate with p-EMT score.

(D) Volcano plot depicts gene expression differences between malignant-basal TCGA tumors with multiple LNs versus those without positive LNs. p-EMT genes (red) have increased expression, while epithelial differentiation genes (green) have decreased expression in metastatic tumors.

(E) Model of the in vivo p-EMT program associated with invasion and metastasis in malignant-basal HNSCC tumors.

See Figure S7.