Seventy-six-megahertz solid-state 13C CPMAS spectra of the
SHaPrP(89–143) peptide, acquired at a spinning speed of 10 kHz.
(A) Spectrum of the wt SHaPrP(89–143) peptide dried
from water. (B) Spectrum of the mutant SHaPrP(89–143,
3AV) peptide dried from water. (C) Spectrum of mutant
SHaPrP(89–143, 3AV) peptide after conversion to an aggregated form by
precipitation from acetonitrile/water. The lines superimposed over
the resonances for V114, A119, and A132 mark the β-sheet chemical
shifts.