In vivo forearm results using square wave patterns and MSE. (a) Calibrated reflectance maps extracted at DC, fundamental (), second, and third harmonic frequencies. The third harmonic image is corrupted by noise. (b) Mean raw and calibrated reflectance values from forearm ROI (black box) at DC, fundamental, second, and third harmonics. Calibrated reflectance values in the ROI agree with conventional SFDI to within 0.1%, 0.3%, 0.5%, and 4.2%, respectively. (c) and maps derived using diffusion model fit and DC, fundamental, and second harmonic reflectance maps. Mean and agree to within 0.5% and 1.0%, respectively.