Fig. 2. Optimization of incubation temperature and reaction time for the Mycobacterium paratuberculosis recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay. (A) Amplification performance of RPA-LFD assay was effective within the range of 35℃ to 45℃. (B) Determination of optimum reaction time. After 10 min of amplification reaction, the test line was clearly visible on the LFD strip; NC, negative control (DNase-free water).
