Fig. 3. Specificity of the recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) assay. The DNA extracted was detected from six strains Mycobacterium and five strains of other bacterial pathogens causing cattle diarrhea; no cross-reactions were detected. Lane 1, Mycobacterium avium subsp. paratuberculosis; Lane 2, Mycobacterium bovis; Lane 3, Mycobacterium tuberculosis; Lane 4, Mycobacterium avium; Lane 5, Mycobacterium phlei; Lane 6, Mycobacterium smegmatis; Lane 7, Escherichia coli O157:H7; Lane 8, Salmonella typhimurium; Lane 9, Campylobacter jujuni; Lane 10, Clostridium perfringens; Lane 11, Leptospira interrogans; NC, negative control (DNase-free water).