Fig. 4. Comparison of sensitivities of the recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) and quantitative polymerase chain reaction (qPCR) assays. Molecular sensitivity test results of the two assays obtained by using 10-fold serially diluted DNA as a template. (A) Results by qPCR. (B) Result by RPA-LFD. Lanes 1–8, a gradient dilution Mycobacterium paratuberculosis plasmid standard DNA from 4 × 106 to 4 copies per microliter; NC, negative control (DNase-free water).
