Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 26;9:558. doi: 10.3389/fimmu.2018.00558

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Hair, Enos, Krishna and Cunnion.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

PA-dPEG24 inhibition of myeloperoxidase (MPO)-initiated neutrophil extracellular trap (NET) formation with human neutrophils (PMN) assayed by fluorescence microscopy and PicoGreen quantitation of free DNA. The first row shows unstimulated neutrophils, the second row shows neutrophils stimulated with MPO and hydrogen peroxide (H₂O₂), the third row shows neutrophils stimulated with MPO + H₂O₂ in the presence of PA-dPEG24 [peptide inhibitor of complement C1 (PIC1)], and the fourth column shows neutrophils incubated with PA-dPEG24 (PIC1) only. The first column shows slides probed with DAPI to visualize DNA. The second column shows slides probed with anti-MPO antibody. The graph shows PA-dPEG24 inhibition of NET generation by human neutrophils stimulated with MPO + H₂O₂ assayed by PicoGreen. Data are means of (n = 3) independent experiments ± SEM.