Table 1.

Primers used for polymerase chain reaction (PCR), rapid amplification of cDNA ends (RACE) PCR, gene expression in tissues/organs, and quantitative real-time PCR (qPCR) of the Dual Domain Carbonic Anhydrase (DDCA) and α-Tubulin from the ctenidium of Tridacna squamosa.

Gene	Primer type	Primer sequence (5′−3′)
DDCA	PCR	Forward: CAAAGATGTCTGGAGGTGG
		Reverse: TCCAACAACTGCTAATCC
	5′-RACE	CAGTCAGCAGGCATTGTATTGGCGAA
	3′-RACE	CACACTCACAACGGCAGGAAATACCC
	Gene expression	Forward: CTATCCATGAATTTCGCCA
		Reverse: TATTTCCTGCCGTTGTGAG
	qPCR	Forward: GATACCAACCAGCCCATCAC
		Reverse: GAAGTCGTAAGCTCTACCCTG
α-Tubulin	qPCR	Forward: GTGCCAAAGGATGTCAATGTC
		Reverse: CTTAGCCATATCTCCGCCTG