Table 3.
Primer sequences and properties used in RT-qPCR study of F. columnare.
| Primer | Sequence | Amplicon Length | Tm (°C) | Efficiency (%) | Reference |
|---|---|---|---|---|---|
| FC_gap1_fwd | ACCATCCCAAACAGGAGCCGC | 98 | 56 | 105.7 | Penttinen et al., 2016 |
| FC_gap1_rev | CGTCTGCTGTAGGTACGCGCA | Penttinen et al., 2016 | |||
| FC_glyA_fwd | CCAAACCCTTGGGGCTATACAACCC | 98 | 60 | 102.8 | Penttinen et al., 2016 |
| FC_glyA_rev | AGAGGGCCTCCTTGATTACCTGGAA | Penttinen et al., 2016 | |||
| FC_gldG_fwd | AGCAGAAGCAGTGATGCAGCA | 125 | 58 | 100.95 | This study |
| FC_gldG_rev | TGCCTTTGTAGGTAGCAATAGCCCA | This study | |||
| FC_gldH_fwd | CTTTGAAAACGGATGGCC | 221 | 56 | 99.15 | Klesius et al., 2010 |
| FC_gldH_rev | CTTGCCCCATAAGACTTCC | Klesius et al., 2010 | |||
| FC_gldL_fwd | GCAAGCGCTATGCTTATTGCTGGT | 131 | 58 | 101.4 | This study |
| FC_gldL_rev | GCAGTTGGTTGTCCCCCTGCT | This study | |||
| FC_sprA_fwd | GCAGAAAATGTTTGGCCCGT | 162 | 60 | 99.95 | This study |
| FC_sprA_rev | ACCGGCAGTTGCTCCATTAT | This study | |||
| FC_sprB_fwd | ACCAGCTGCTCCATGGTCAACTAC | 157 | 60 | 100.1 | This study |
| FC_sprB_rev | CGAAGGTGTCGTAGGGGCCG | This study | |||
| FC_sprE_fwd | AGCCGTGCAGAAGATAAAGC | 151 | 60 | 100.8 | This study |
| FC_sprE_rev | ACGCTTCTAATGCGGGTACAA | This study | |||
| FC_sprF_fwd | AGTCGTCAAATGGGGGCTAA | 148 | 60 | 99.65 | This study |
| FC_sprF_rev | TCACGCTTCCATCAAAGGTT | This study | |||
| FC_sprT_fwd | AACCAGGACTGCATTACGGA | 144 | 60 | 101.1 | This study |
| FC_sprT_rev | GCTTGATGTTACCTGTGCGTT | This study | |||
| FC_porV_fwd | GTGCCAACTCCTAAAACAGCC | 152 | 60 | 96.85 | This study |
| FC_porV_rev | AAACCTCCTGGAGCATCACC | This study | |||