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. 2018 Mar 26;8:208. doi: 10.3389/fgene.2017.00208

Figure 1.

Figure 1

Increased expression levels of both p21 and FAS mediated by p53 in MALAT1-knockdown cells. (A,B) Real-time PCR analyses were performed to assess the effects of knockdown of p53 by p53 siRNA 1 (sip53(1)) and p53 siRNA 2 (sip53(2)) (A) or inhibition of p53 by PFT-α in the presence of doxorubicin (B) on the expression of p21, FAS and DNAJC15 genes in MALAT1-knockdown cells. Relative mRNA expression levels are presented as ratios to the level of that in control cells. Data are presented as means ± standard deviation (SD) of three independent experiments (***P < 0.001, two-way ANOVA followed by Bonferroni t-test as post-hoc test), except for DNAJC15, which shows the average of duplicate experiments. (C) p53-null H1299 cells were transfected with MALAT1 siRNA, and p21 and FAS expression levels were analyzed by real-time PCR. Values represent the means ± SD of duplicate measurements.