FIG 3.

Lipolysis pathway is impaired in CDP138 knockout mice. WT and KO female mice at 12 weeks of age were cold challenged (CC) for 2 h or fed with either NCD or an HFD for 10 weeks. Total tissue lysates from brown adipose tissue (BAT), subcutaneous inguinal white adipose tissue (iWAT), and gastrocnemius (muscle) were used for measuring cAMP levels with an ELISA kit (A) and for immunoblotting with antibodies against phospho-HSL (Ser660, a PKA phosphorylation site), HSL, ATGL, and Vps10p-tail interactor 1a (Vti1a) (B and C). Quantitative data for immunoblotting are presented as arbitrary units relative to the intensity of the band from the WT group. All data are presented as means ± SEM (n = 5 in each group; P < 0.05 [*] and P < 0.01 [**] for WT group versus KO group).