FIG 4.

Deletion of CDP138 inhibits thermogenesis in brown adipose tissue. WT and KO male mice (10 weeks old) were challenged with cold (4°C for 2 h). (A) Mouse body temperatures were measured before and after the cold challenge with a mouse rectal thermometer. (B and C) Representative images of H&E staining (B) and immunofluorescence staining (C) of UCP-1 in BAT from WT and KO mice. Scale bars, 100 μm. (D and E) Immunoblot analysis and quantification (presented as arbitrary units) of UCP-1 protein expression in BAT from WT and KO mice. CDP138 and Vti1a were used as markers of loading control. (F) Gene expression levels of factors related to mitochondrial function. (G and H) Fatty acid oxidation (FAO) rates of BAT (G) and skeletal muscle (H) homogenates from WT and KO mice under NCD conditions were determined by measuring [1-¹⁴C]palmitate oxidation. All data are presented as means ± SEM (n = 6 in WT group and 5 in KO group; P < 0.05 [*] and P < 0.01 [**] for WT versus KO mice).