FIG 6.

CDP138 is colocalized with sympathetic marker tyrosine hydroxylase (TH) in inguinal fat and is involved in the regulation of norepinephrine levels in fat tissue. (A) Formaldehyde-fixed inguinal fat sections from 20-h cold-challenged mice were used for immunofluorescence staining with antibodies against TH and CDP138 as described in Materials and Methods. Arrows indicate colocalization of CDP138 and TH in iWAT. (B) TH expression in iWAT from WT and CDP138 KO mice fed a chow diet. (C) Norepinephrine levels in iWAT after cold challenge for 20 h. n = 8 for each group. *, P < 0.05 for WT versus KO mice. (D) CDP138 expression in iWAT from C57BL/6 mice fed a chow diet (NCD) or an HFD (60 kcal% fat) for 16 weeks. *, P < 0.05 for NCD versus HFD; n = 6 for each group.