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FPLC Chromatograms of the one-pot syntheses of UTP and CTP. (a) The synthesis of UMP is nearly complete at 5 hours. At this point, the components to synthesize UTP are added, completing its synthesis at 12 hours. (b) The synthesis of CTP is nearly complete at 4 hours. Both UTP/dATP and CTP/dADP have identical elution volumes, hence the peaks do not appear to be completely depleted, as dATP and dADP are both in large excess in the reaction mixture.
